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Essential Molecular Biology Vol 2

Second Edition

Edited by Terry Brown

Price: £47.50 (Paperback)
ISBN-13: 978-0-19-963644-0
Publication date: 15 November 2001
310 pages, 11 line drawings, 9 halftones, 246x189 mm
Series: Practical Approach Series number 255
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Description

Fully revised and updated, with some chapters rewritten
Clear theoretical and practical guide to the fundamental techniques of gene cloning

The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements.

Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.

Readership: The two Essential Molecular Biology Practical Approach books are aimed at the absolute beginner at cloning genes.

Contents

1. Strategies for research in molecular biology , T.A. Brown

2. Construction of genomic libraries in lambda and cosmic vectors , Anna-Maria Frischauf

3. Construction of a cDNA library , Madan K. Bhattacharyya

4. Nucleic acid labelling and detection , C.R.Mundy, M. W.Cunningham and C.A.Read

5. Immobilization of nucleic acids and hybridization analysis , N.J.Dyson

6. DNA sequencing , T.A.Brown

7. The polymerase chain reaction , T.A.Brown

8. Protein expression in Escherichia coli , John M. Ward

9. Transcript mapping , Colin P.Smith

1. Appendices Health hazards and safety procedures

2. Equipment for molecular biology research

3. Important Escherichia coli strains

4. Recipes and general procedures

5. Restriction endonucleases

6. DNA and RNA modification enzymes

7. List of suppliers' addresses

Authors, editors, and contributors

Edited by Terry Brown, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology

Contributors:Madan K. Battacharyya, T. A. Brown, M. W. Cunningham, N. J. Dyson, Anna-Marie Frishauf, C. R. Mundy, C. A. Read, Colin P. Smith, John M. Ward

Links to web resources and related information

More in the same subject area:
Food & beverage technology
Molecular biology
Biochemistry
Cellular biology
Genetics (non-medical)

The specification in this catalogue, including without limitation price, format, extent, number of illustrations, and month of publication, was as accurate as possible at the time the catalogue was compiled. Occasionally, due to the nature of some contractual restrictions, we are unable to ship a specific product to a particular territory. Jacket images are provisional and liable to change before publication.