| Description | | - Covers purification of proteins from common sourcesDescribes the purification of inclusion bodies and fusion proteins and purification for crystallographyWritten by practical scientists for practical scientists
| |
Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the
introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and
rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques
focuses on unit operations and
analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications
describes purification strategies from common
sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These
books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
|
Readership: All scientists in the biotechnology industry, pharmaceutical industry, or academic research who need to purify proteins.
| Contents |
Fusion Protein Purification Methods
Initial Purification of Inclusion Bodies
Purification for Crystallography
Purification from Mammalian Cell Culture
Purification from Microbial Cell Culture
Purification from Milk
Purification from Animal Tissue
Protein Purification from Plant Sources
|
| Authors, editors,
and contributors |
Edited by Simon Roe, AEA Technology Products and Systems, Biosciences, Harwell
|
The specification in this catalogue, including without
limitation price, format, extent, number of illustrations,
and month of publication, was as accurate as
possible at the time the catalogue was compiled.
Occasionally, due to the nature of some contractual restrictions, we
are unable to ship a specific product to a particular territory.
Jacket images are provisional and liable to change before publication.
|
VIEW BASKET
