| Reviews |
| - ''Stephen A.Baldwin has performed a service to the scientific community in compelling these technical approaches to membrane transport studies' Journal Cell Science' -
|
| Description | | - New design
- Up-to-date information written by leading experts in the field
- Comprehensive collection of over 90 protocols
- Describes approaches that can be implemented in any laboratory
- Very well illustrated
| | Membrane Transport
is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary
technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of
genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.
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Readership: Postgraduate and postdoctoral biochemists/molecular biologists/cell biologists.
| Contents |
Assay of membrane transport in cells and membrane vesicles
Reconstitution of membrane proteins: the Ca2+-ATPase of sarcoplasmic reticulum
The Xenopus
oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins
Expression of foreign transport proteins in yeast
Baculovirus-mediated overexpression of transport proteins
The amplified expression, identification purification, assay and properties of hexahistidine-tagged bacterial membrane transport proteins
Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters
Detection and analysis of glucose transporters using photolabelling techniques
Computer prediction of transporter topology and structure
Two-dimensional crystallization of membrane proteins
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| Authors, editors,
and contributors |
Edited by Stephen A. Baldwin, School of Biochemistry and Molecular Biology, University of Leeds
| | Contributors:Stephen A. Baldwin, School of Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT; Carol E. Cass, Cross Cancer Institute, Edmonoton, Alberta, Canada T6G 1Z2; Richard J. Cogdell, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland G12 8QQ; Andreas Engel, Maurice E. Muller-Institut fur
Mikroskopische Strukturbiologie am Biozentrum, Universitat Basel, CH-4056 Basel Klingelbergstrasse 70, Switzerland; Alison K. Gillingham, School of Biology and Biochemistry, South Building, University of Bath, Claverton Down, Bath BA2 7AY; Makoto Hashimoto, School of Biology and Biochemistry South Building, University of Bath, Claverton Down, Bath BA2 7AY; Peter J.F. Henderson, School of
Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT; Bernard Heymann, Maurice E. Müller-Institut für Mikroskopische Strukturbiologie am Biozentrum, Universität Basel, CH-4056, Basel, Klingelbergstrasse 70, Switzerland; Geoffrey D. Holman, School of Biology and Biochemistry, South Building, University of Bath, Claverton Down, Bath, BA2 7AY; Rong-I Hong, Laboratory of Molecular
Biophysics, University of Oxford, Rex Richards Building, South Parks Road, Oxford OX1 3QU; Tina D. Howard, Division of Biochemistry & Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ; Simon M. Jarvis, Dept. of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ; François Koumanov, School of Biology and Biochemistry, South Building,
University of Bath, Claverton Down, Bath BA2 7AY; Anthony G. Lee, Dept. of Biochemistry, School of Biological Sciences, University of Southampton, Medical and Biological Sciences Building, Bassett Crescent East, Southampton, SO9 3TU; Gary J. Litherland, Dept. of Anatomy and Physiology, Old Medical School, University of Dundee, Small's Wynd, Dundee DD1 4HN; Katherine E. Mcauley-hecht, Division of
Biochemistry & Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland; John O'Reilly, School of Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT; Bert Poolman, Dept. of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerlaan 30, 9751 NN Haren, The Netherlands; Philippe Ringler, Maurice E.
Müller-Institut für Mikroscopische Strukturbiologie am Biozentrum, Universität Basel, CH-4056 Basel, Klingelbergstrasse 70, Switzerland; Nicholas G. Rutherford, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT; Neil M. Sanderson, School of Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT; Mark S.P. Sansom, Lab. Of Molecular Biophysics, University
of Oxford Rex Richards, Building, South Parks Road, Oxford OX1 3QU; Norbert Sauer, Lehstuhl Botanik II, Universität Erlangen, Staudtstrasse 5, D-91058 Erlangen, Germany; Jurgen Stolz, Lehrstuhl Botanik II, Universität Erlangen, Staudstrasse 5, D-91058 Erlangen, Germany; Adrian R. Walmsley, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow
G12 8QQ; Alison Ward, School of of Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT; Sylvia Y. M. Yao, Dept. of Physiology , Faculty of Medicine, 7-55 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7; James D. Young, Department of Physiology, Faculty of Medicine, 7-55 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada
T6G 2H7 |
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