| Reviews |
| - '"This book is very useful not only for immunologists but also for amny specialists in chemistry and biology and is certainly recommendable" Immunological Investigations' -
|
| Description | | - Up-to-date techniques written by uniquely qualified contributors
- Contains over 75 protocols
| | Immunoassays
is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents,
standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the
calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
|
Readership: Researchers in: universities, hospitals, research institutes, governments and regulatory agencies, service and manufacturing industries.
| Contents |
Preface
Protocol List
Abbreviations
1.
Analysis by specific binding
,
James P. Gosling
2.
Raising antibodies
,
Marian M. Kane and John N. Banks
3.
Choosing and characterizing antibodies
,
Anthony Forde and John Coley
4.
Labels and endpoints
,
Wajdi Abdul-Ahad and Martin Brett
5.
Solid-phase reagents
,
Robert S. Matson
6.
Standards and immunogens
,
M. Quasim Chaudry
7.
Optimization
,
Eric Ezan and Jacques Grassi
8.
Validation
,
Peter O'Fegan
9.
Data processing
,
Barry Nix and David Wild
10.
Quality assurance
,
John Seth
A1. Suitable reagents and other requisites
A2. List of suppliers
Index
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| Authors, editors,
and contributors |
Edited by James P. Gosling, Department of Biochemistry and National Diagnostic Centre, National University of Ireland, Gallaway
|
The specification in this catalogue, including without
limitation price, format, extent, number of illustrations,
and month of publication, was as accurate as
possible at the time the catalogue was compiled.
Occasionally, due to the nature of some contractual restrictions, we
are unable to ship a specific product to a particular territory.
Jacket images are provisional and liable to change before publication.
|
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