| Description | | - The book emphasizes practical aspects of using differential display illustrated with over 100 protocols
- Provides real examples of differential display studies
- Provides practical advice about the use of differential display in the nervous system
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One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentially expressed in various tissues and cell types, throughout ontogenetic development and in pathological
processes, will go a long way towards understanding the function of all these 'new' genes and their protein products. Differential Display
explains in detail how to perform the technique of RT-PCR Differential Display in various kinds of experimental biological systems. It also examines this technique in the context of other methods of studying differential gene expression such as
subtractive hybridisation and the use of high-density gene microarrays combined with hybridisation techniques and automatic image analysis.
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Readership: Postgraduate biological scientists working in academia and industry. Molecular biologists, pharmacologists, neuroscientists, microbiologists, oncologists, developmental biologists and plant scientists.
| Contents |
An introduction to differential display and related techniques
Recent advances in fluorescent differential display
Practical aspects of the experimental design for differential display of transcripts obtained from complex tissues
RAP-array : expression profiling using reduced complexity probes for cDNA arrays
The use of RT-PCR differential display in single-celled organisms and plant tissues
A modified approach for the efficient display of 3' - end restriction fragments of cDNAs
Cloning of differentially expressed brain cDNAs
Use of suppression subtractive hybridization strategy to identify differential gene expression in brain ischemic tolerance
Studying gene expression profiles in specialized brain regions by microsage
Construction and applications of gene microarrays on nylon membranes
Subtracted differential display and antisense approaches to analyzing brain signalling
Differential display analysis of memory-associated genes
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| Authors, editors,
and contributors |
Edited by Ron A. Leslie, SmithKline Beecham Pharmaceuticals, Harlow and
Harry A. Robertson, Dalhousie University, Halifax
| | Contributors:David Chambers, Nicole Datson, John B. Davis, Christine M. Debouck, Eileen M. Denovan-Wright, Giora Z. Feuerstein, Krista L. Gilby, Julie Gray, Susan E. Howlett, Tzung-Fu Hsieh, A-Min Huang, Peter Jenner, Barbara Jung, Paul S. Kayne, Eminy H. Y. Lee, Ronald A. Leslie, Karen Lowe, Ivor Mason, Francoise Mathieu-Daude, Michael McClelland, Andrew D. Medhurst, Jeffrey L. Mooney, Peter E.
Newburger, Richard A. Newton, Michael L. Nuccio, Shawn P. O'Brien, Harold A. Robertson, Julian A. Shale, Y.V.B.K. Subrahmanyam, Terry L. Thomas, Thomas Trenkle, G. R. Uhl, Erno Vreugdenhil, X.B. Wang, Xinkang Wang, Sherman M. Weissman, John Welsh, Sigeru Yamaga |
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limitation price, format, extent, number of illustrations,
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possible at the time the catalogue was compiled.
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