| Reviews |
| - '" ... chapters are well organized and the protocols are clearly highlighted ... a good starting point for anybody interested in a 'practical approach' to the topic of gene expression." Trends in Biochemical Sciences' -
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| Description | | - New design.
- Over 100 protocols.
- Written by leading experts in the field.
- Suitable for specialists and non-specialists.
- Covers the most successful methods in functional genomics.
| | With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides a comprehensive treatment of the range of methods available for gene and protein expression profiling in a
variety of systems. These include large-scale methods, such as cDNA microarrays, serial analysis of gene expression (SAGE) and proteomics, and also methods suitable for non-specialist laboratories, such as differential display and suppression subtraction hybridization. In all cases the chapters have been written by the developers of these methods or experienced users and include detailed
protocols to facilitate the introduction of these methods to the readers' laboratories. |
Readership: Research scientists, clinicians and clinical pathologists in biomedical science, involved in gene & protein expression profiling.
| Contents |
Functional Genomics: approaches and methodologies
Construction and Screening of a Subtractive cDNA Library
Differential Display Analysis of Alterations in Gene Expression
Representational Difference Analysis of cDNA
Identification of Differentially Expressed Genes by Suppression Subtractive Hybridization (SSH)
Gene Expression Analysis by cDNA Microarrays
SADE: A Microassay for Serial Analysis of Gene Expression
Analysis of Gene Expression in Single Cells Using Three Prime End Amplification (TPEA) PCR
Analysis of Gene Expression by 2-D Gel Electrophoresis
Proteome Research: methods for protein characterization
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| Authors, editors,
and contributors |
Edited by Stephen Hunt, Department of Anatomy, University College London and
Frederick . Livesey, Department of Genetics, Harvard Medical School
| | Contributors:Armen N. Akopian, Department of Biology, University College London; Andy Baxevanis, Cancer Genetics Branch, National Human Genome Research Institutes, USA; Michael L. Bittner, Cancer Genetics Branch, National Human Genome Research Institutes, USA; Yidong Chen, Cancer Genetics Branch, National Human Genome Research Institutes, USA; Lydie Cheval, Department de Biologie Cellulaire et
Moleculaire, Service de Biologie Cellulaire, France; Yong-Jing Cho, Vanderbilt Cancer Centre, Department of Cell Biology, Vanderbilt University School of Medicine, USA; Sejal Desai, Gene Cloning and Analysis Group, CLONTECH Laboratories, USA; Luda Diatchenko, Gene Cloning and Analysis Group, CLONTECH Laboratories, USA; Alistair K. Dixon, Cambridge; Ed R. Dougherty, Cancer Genetics Branch, National
Human Genome Research Institutes, USA; Jean-Marc Elalouf, Department de Biologie Cellulaire et Moleculaire, Service de Biologie Cellulaire, France; Tom Freeman, Sanger Centre, Cambridge; Jason Hill, Gene Cloning and Analysis Group, CLONTECH Laboratories, USA; M. Hubank, University of Manchester; Ian Humphery-Smith, Department of Pharmaceutical Proteomics, University of Utrecht, The Netherlands;
Stephen P. Hunt, Department of Anatomy and Developmental Biology, Medawar Building, University College London; Kevin Lee, Cambridge; Peng Liang, Vanderbilt Cancer Center, Department of Cell Biology, Vanderbilt University School of Medicine, USA; F. J. Livesey, Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, USA; Paul S. Meltzer, Cancer Genetics Branch, National
Human Genome Research Institutes, USA; Spyro Mousses, Cancer Genetics Branch, National Human Genome Research Institutes, USA; Peter J. Richardson, University of Cambridge.; D.G. Schatz, Howard Hughes Medical Institute and Section of Immunology, Yale University School of Medicine, USA; Paul D. Siebert, Gene Cloning and Analysis Group, CLONTECH Laboratories, USA; A. M. Tolkovsky, Department of
Biochemistry, University of Cambridge; Stephanie Trelogan, CLONTECH Laboratories, USA; Jeffrey M. Trent, Cancer Genetics Branch, National Institutes of Health, USA; Bérangére Virlon, Départment de Biologie Cellulaire et Moléculaire, Service de Biologie Cellulaire, France; Malcolm A. Ward, Cell Mapping Project, Glaxo Wellcome Research and Development, UK; John N. Wood, Department of Biology,
University College London |
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limitation price, format, extent, number of illustrations,
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