| Reviews |
| - 'The chapters are well written, with sufficient background detail and a step-by-step guide to each technique. Overall, this will be a useful manual for any plant molecular biology laboratory.' - Microbiology Today
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| Description | | Volume Two focuses on experimental approaches for studies on gene expression, gene product analysis, with the final section devoted to emerging technologies. Topics covered include a range of techniques for transcript analysis, including In situ Hybridization and DNA microarrays. DNA-protein interaction methods are also covered in detail. Inducible gene expression in plants as well as
expression and analysis of recombinant proteins, and analysis of protein import into chloroplasts are covered as well as techniques for fractionation of plant tissue for biochemical analyses and the study of protein-protein interactions with the yeast two-hybrid system. A range of approaches for using antibodies as tools are also described including the use of antibody phage display libraries.
The final section on emerging technologies describes methodologies for calcium imaging and for the spatial and temporal analysis of reporter genes such as luciferase and green fluorescent protein. The final area covers a range of experimental procedures for moss, which is emerging as a new model organism. |
Readership: Graduate students and researchers in molecular biology and plant sciences.
| Contents |
Part 1: Gene Expression
1.
Transcript analysis
2.
In situ hybridization
3.
DNA microarrays for gene expression analysis
4.
DNA-protein interactions
5.
Inducible gene expression in plants
Part 2: Gene Product Analysis
6.
Expression of recombinant proteins
7.
Import of proteins into isolated chloroplasts and thylakoid membranes
8.
Fractionation of plant tissue for biochemical analysis
9.
Studying protein-protein interactions with the yeast two-hybrid system
10.
Antibody techniques
11.
Antibody phage display libraries
Part 3: Emerging Technologies
12.
Spatial and temporal measurements of calcium ions in living plant cells
13.
Reporter genes for plant cells
14.
Moss gene technology
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| Authors, editors,
and contributors |
Philip M Gilmartin, Director, Centre for Plant Sciences, University of Leeds and
Chris Bowler, Head, Laboratory of Molecular Plant Biology, Stazione Zoologica, Napoli, Italy
|
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limitation price, format, extent, number of illustrations,
and month of publication, was as accurate as
possible at the time the catalogue was compiled.
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