| Description | | - Revised and updated version of a highly popular book
- Provides techniques that wide applications throughout biomolecular sciences
- Combines general principles with practical methods
| Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and
the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.
Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along
with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the
determination of active site concentration.
This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences. |
Readership: Researchers in biochemistry and molecular biology, and all life sciences
| Contents |
1.
Principles of enzyme assay and kinetic studies
,
Keith F Tipton
2.
Photometric assays
,
Robert A John
3.
Radiometric assays
,
Kelvin T Hughes
4.
High performance liquid chromatographic assays
,
Shabih E H Syed
5.
Electrochemical assays: the oxygen electrode
,
J B Clark
6.
Electrochemical assays: the nitric oxide electrode
,
R D Hurst and J B Clark
7.
Electrochemical assays: the pH-stat
,
Keith Brocklehurst
8.
Enzyme assays after gel electrophoresis
,
Gunter M Rothe
9.
Techniques for enzyme extraction
,
Nicholas C Price and Lewis Stevens
10.
Determination of active site concentration
,
Mark T Martin
11.
High throughput screening - considerations for enzyme assays
,
David Hayes and Geoff Mellor
12.
Statistical analysis of enzyme kinetic data
,
Athel Cornish-Bowden
|
| Authors, editors,
and contributors |
Edited by Robert Eisenthal and
Michael Danson, both in the Department of Biology and Biochemistry, University of Bath
|
The specification in this catalogue, including without
limitation price, format, extent, number of illustrations,
and month of publication, was as accurate as
possible at the time the catalogue was compiled.
Occasionally, due to the nature of some contractual restrictions, we
are unable to ship a specific product to a particular territory.
Jacket images are provisional and liable to change before publication.
|
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