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Flow Cytometry
A Practical Approach

Third Edition

Edited by Michael G. Ormerod

Price: £47.50 (paper)
ISBN-13: 978-0-19-963824-6
Publication date: 18 May 2000
296 pages, 99 line illus, 246x189 mm
 Series: Practical Approach Series number 229
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Reviews
  • 'This book will be useful to those people who wish to begin exploring the fascinating and attractive world of flow cytometry and also to those who need to update their knowledge of flow cytometry protocols in mammalian cell biology. It is also an excellent reference book on flow cytometry for use in the laboratory.' - Cell Biology International

Description
  • Updated volume with an additional apoptosis chapter
  • Invaluable to both beginner and expert
  • New design
  • Over 50 protocols and 99 figures.
Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

Readership: Research and clinical laboratories.

Contents
Preface
List of Protocols
Abbreviations
1. Introduction to the principles of flow cytometry , Nigel P. Carter and Michael G. Ormerod
2. Fluorescence and fluorochromes , Michael G. Ormerod
3. Preparing suspensions of single cells , Michael G. Ormerod
4. Flow sorting , Nigel P. Carter and Michael G. Ormerod
5. Immunofluorescence of surface markers , Michael R. Loken, Cherie I. Green, and Denise A. Wells
6. Analysis of DNA - general methods , Michael G. Ormerod
7. Further clinical applications , Terry Hoy, et al.
8. Quality assurance in the clinical laboratory , Jan W. Gratama and David Barnett
9. Measurement of cytoplasmic and nuclear antigens , Jorgen K. Larsen
10. Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method , George D. Wilson
11. Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method , Michael G. Ormerod and Martin Poot
12. Chromosome analysis and sorting by flow cytometry , Derek C. Davies, Simon P. Monard, and Bryan D. Young
13. Intracellular ionised calcium, magnesium, membrane potential and pH , Peter S. Rabinovitch and Carl H. June
14. Flow cytometry in the study of apoptosis , Michael G. Ormerod
15. Further applications to cell biology , Michael G. Ormerod
A1. Safety Procedures
A2. Flow cytometers and software
A3. List of suppliers
Index

Authors, editors, and contributors


Edited by Michael G. Ormerod


Links to web resources and related information
More in the same subject area:
Immunology
Cellular biology

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